Sad 2 is one of several morose themes that plays randomly in SimAnt when something bad happens. This can include being killed by a spider, ant lion, or red ant, as well as disasters like being sucked up into the lawn mower or sprayed by insecticide, and even just the general game over theme. It was composed either by Matthew Berardo, Steve Hales, Jack Thorton, or Chris Schardt.
The title comes from the song's file name and the meta data in it.
This is the original version of the song.
The song is index 27 in the SOUND.DAT file and "SAD2.MID" is the title in the MIDI meta data.
The Windows version of the song plays much slower than the original DOS version. There appears to be a bug in the conversion process. Although the entire song is supposed to be played on an instrument called "strings," the first note in the Windows port of the song is played on piano.
The song's file name is SAD2.MID, and "SAD2.MID" is its title in the MIDI meta data.