Each of the Boss Stages in Super Noah's Ark 3D (Level 1-3, Level 2-4, Level 3-4, Level 4-5, Level 5-6 and Level 6-7) play this track. It has a more sinister sound than the rest of the game's music. The song is the same for both the DOS and SNES releases, but the different audio hardware plays them each with a distinct sound.
Super Noah's Ark 3D (SNES)
The song plays on every boss stage and is output to S-SMP chip.
Super Noah's Ark 3D (DOS)
In the DOS release, the song is output to OPL2 and plays in the same boss stages (E1M3, E2M4, E3M4, E4M5, E5M6, and E6M7) as in the SNES release.